Bio-Rad Nuvia™ S Media User Manual
Page 8
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Table 2. Nuvia S media specifications.
Type of exchanger
Strong cation
Functional group
–SO
3
–
Total ionic capacity
90–150 µeq/ml
Dynamic binding capacity
*
≥110 mg/ml (300 cm/hr)
Shipping counterion
Na
+
Median particle size
85 ± 15 µm
Recommended linear flow rate
**
50–600 cm/hr
Chemical stability
1.0 N NaOH (20°C)
Up to 1 week
0.1 N NaOH (20°C)
***
Up to 5 yr
pH stability
***
2–14 short-term
4–13 long-term
Shipping
20% ethanol + 0.1 M NaCl
Regeneration
1–2 M NaCl
Sanitation
0.5–1.0 N NaOH
Storage
20% ethanol or 0.1 N NaOH
*
10% breakthrough capacity determined with 4.5 mg/ml human IgG in
40 mM NaOAc, 30 mM NaCl, pH 5.0.
**
Elution flow rates <150 cm/hr minimize viscosity-induced backpressure.
***
Data derived under accelerated conditions at 60ºC.