Bio-Rad Nuvia™ S Media - Manual (Page 20)

16
An appropriate starting point for purifying
samples is a linear gradient of 0–0.4 M NaCl
spanning 1–20 column volumes at 120 cm/hr,
0.5 ml/min for the 1 ml cartridge and
2.5 ml/min for the 5 ml cartridge. Separation
can be optimized by changing the gradient
profile.
Table 4. Common ion exchange buffers.
Cation Buffer Range
Acetic acid 4.8–5.2
Citric acid 4.2–5.2
HEPES 6.8–8.2
Lactic acid 3.6–4.3
MES 5.5–6.7
MOPS 6.5–7.9
Phosphate 6.7–7.6
PIPES 6.1–7.5
TES 6.8–8.2
Tricine 7.8–8.9