Bio-Rad Nuvia™ S Media - Manual (Page 22)

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Section 5
Cleaning-in-Place (CIP)
and Sanitation
If a column no longer yields reproducible
results, the media may require thorough CIP
and sanitation to remove strongly bound
contaminants. Acceptable CIP agents include
25% acetic acid, 8 M urea, 1% Triton X-100,
6 M potassium thiocyanate, 70% ethanol,
30% isopropyl alcohol, 1 N NaOH, and 6 M
guanidine hydrochloride.
1. Sanitize the support in the column with
2–4 bed volumes of 1.0 N NaOH at
50–100 cm/hr while maintaining a contact
time of at least 40 min.