1 autoclaving, 2 storage – Bio-Rad Nuvia™ S Media User Manual

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2. To reequilibrate the column, wash it with

2–4 bed volumes of 0.5–2 M NaCl solution
(containing 50–100 mM buffer).

3. If lipid removal is required, wash the

column with a 20–70% ethanol solution at
50 cm/hr.

5.1 Autoclaving
Bio-Scale

Mini cartridges are not

autoclavable.

5.2 Storage
After washing the cartridges with
deionized water, Bio-Scale Mini ion
exchange cartridges should be purged,
stored in 20% ethanol solution, and
capped for extended storage.

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