Bio-Rad Nuvia™ S Media - Manual (Page 18)

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3. Wash with degassed high-salt buffer for
5 min.
4. Equilibrate with low-salt buffer for 5 min.
5. Reduce the flow rate to the rate that will be
used in the purification protocol.
4.1 Sample Preparation
Correct pH and ionic strength are necessary
for consistent and reproducible results.
Sample can be exchanged into the starting
buffer or diluted to the starting buffer
concentration. This can be achieved by
diluting the sample to the ionic strength of the
starting buffer, dialyzing against the starting
buffer, or exchange into the starting buffer.
Buffer exchange can be accomplished using
a number of products (Table 3). The choice of
product will depend on sample volume. Filter
all samples through a 0.45 µm filter prior to
cartridge application.