Section 4 preparing a cartridge for use – Bio-Rad Nuvia™ S Media User Manual
Page 17
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13
Section 4
Preparing a Cartridge for
Use
Bio-Scale
™
Mini Nuvia
™
cartridges contain
20% ethanol + 0.1 M NaCl (v/v) as the
shipping solution. The fully hydrated support
is ready to use after equilibrating the cartridge
in the buffer of choice. To perform a buffer
exchange, connect the cartridge to a liquid
chromatography system or peristaltic pump
and condition it as follows:
1. Set the pump flow rate to 1–2 ml/min
(240–280 cm/hr) for the 1 ml cartridge or
5–10 ml/min (140–480 cm/hr) for the 5 ml
cartridge.
2. Wash the cartridge with degassed low-salt
buffer for 2 min.
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