Dako Hybridizer - Manual (Page 17)

Dako Hybridizer | Handbook 17
Note: If all 40 program numbers have been used, the “Create a PGM” line in the Main Menu will no
longer appear. An existing program must be edited. See section 5.
Create a Denaturation and Hybridization Program (Denat & Hyb)
Turn unit on and wait for the Main Menu screen. Use the arrow keys to move cursor to “Create a PGM”
and press “Enter” button to accept.
Cursor highlights “Denat & Hyb” line; press “Enter” button to accept.
Hybridizer will advance to the next available program number.
Hybridizer allows creating a program name. Cursor highlights the first name character position. Use the
arrow keys to move through character set and press “Enter” button to accept the characters. All 10
character positions must be filled. Press “Enter” button to accept blank characters. For numeric
characters use keypad 0-9.
Character set: A-Z; 0-9; period, - and blank (“Enter” button or move arrow)
Cursor will advance to “Denat Temp”. With numeric keypad enter a two-digit temperature value in
degrees Celsius (50-99
°C).
Cursor advances to “Denat Time”. With numeric keypad enter a two-digit time value in minutes (0 – 30).
Cursor advances to “Hyb Temp”. With numeric keypad enter a two-digit temperature value in degrees
Celsius (30-70
°C). The instrument allows a temperature of 30°C or ambient temp + 5°C (whichever is
higher) for the lowest hybridization temperature. For room temperature hybridization (ambient temp +
5
°C) enter the two-digit value 00.
Cursor advances to “Hyb Time”. With numeric keypad enter a two-digit time value in hours (0 – 99)
followed by a two-digit value in minutes (0-59).
Display will now show entered program values. Cursor highlights “Enter to Accept” line.
Press “Enter” button to accept the program values; or press “Backspace” button to return to previous
screen to modify program values; or press the “Stop” button to abort.
Note: Accepting the program saves the program by its assigned program number and name.
PGM 01 Name
Enter: Temp Time
Denat: 50°C :00
Hyb: 30°C 00:00
Denat: 82°C :05
Hyb: 45°C 20:00
ENTER to Accept
STOP to Abort