Labconco FreeZone Triad Freeze Dry System 74000 Series - Manual (Page 29)

Chapter 4: Operating Instructions
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compromised and damage could occur to the vacuum pump when
turned on in segment 1.
2.
Set mode to AUTO.
3.
Place the unfrozen samples on the shelf and close the door.
4.
Start the refrigeration system by pressing “RUN/STOP.”
Note: The vacuum pump will run 10 seconds and then turn off
automatically.
Stoppering Vials
The stoppering operation (when desired) is performed after the freeze dry
process is complete and before breaking vacuum. To seal vials, move the
Stoppering control toward the “LOWER” position. This action allows the
diaphragm above the stoppering platen to inflate causing the platen to lower.
The platen will lower until it contacts the vials on the shelf. The rubber
stoppers will be pressed into the vials. Monitor the stoppering process by
looking through the chamber door.
When all of the vials appear to be stoppered, move the Stoppering Control to
the “RAISE” position. This opens the diaphragm to the vacuum pump,
which deflates the diaphragm. Opening the Vacuum Release Control also
deflates the stoppering diaphragm. The Stoppering Control should be left in
the “RAISE” position when not stoppering. The stoppering mechanism is
equipped with anti-tilt switch which will stop the platen from lowering if it
tilts more that 4°: If this happens return the stoppering control valve to
“RAISE” position. This will deflate the diaphragm and allow the platen to
return to the raised position. Determine the cause of the tilt condition before
proceeding.
Vacuum Break/Backfilling
To open the chamber door, the vacuum must be released. To release the
vacuum, move the Vacuum Release Control to the “OPEN” position and shut
off the vacuum pump by pressing the VAC button. The vacuum can also be
released more quickly by opening one of the sample valves.
Air enters the vacuum chamber through the Back Fill Port. When the sound
of air through the Back Fill Port is no longer audible, the chamber door is
ready to open.
To backfill the chamber with a gas, connect a cylinder of the desired gas to
the Back Fill Port. The port will accept 1/8" tubing. The bottle must be
equipped with a regulator set to 15 psi maximum. The gas can be metered
with the Vacuum Release Control and chamber pressure monitored with the
freeze dry system’s vacuum gauge. Allow the vacuum pump to run for a few
minutes while the gas enters to permit the back fill gas to surround the freeze
dried sample. When the vacuum pump is turned off, air will enter the system
through the vacuum break valve located between the vacuum pump and the
collector chamber.