Iris Sample Processing StatSpin® CenSlide 2000 Urine Sediment Analysis System - Manual (Page 15)

Special Topics
OPERATOR’S MANUAL
CenSlide 2000 Urinalysis System Page 3 - 1
This chapter covers special procedures, technical
specifications, and references.
3.0 Staining
The recommended stain for use with the CenSlide 2000 is a
modified Sternheimer-Malbin stain. This stain is commercially
available, call StatSpin for source (800-782-8774).
This stain, when used properly, provides increased contrast
and specific staining of urinary sediment. This makes
recognition of some formed particles, especially casts, easier.
3.0.1 Preparing Censlide Tubes for Centrifugation
1. Label a CenSlide tube with the appropriate patient information.
2. A first morning specimen with an acid pH is recommended
because it is concentrated and more likely to contain
casts than a dilute alkaline afternoon specimen.
Dispense a sample for analysis as follows:
a. Shake the urine collection container sufficiently to
resuspend the particles uniformly in the urine.
b. Remove the lid from the urine collection container and
pour the urine into the open end of a CenSlide tube up to
the line directly above the arrow (5 mL).
3. If requested, insert a chemistry
urinalysis test strip into the CenSlide
tube prior to adding stain, and tilt it
slightly to assure that urine completely
covers the uppermost pad.
4. Shake the stain bottle before
dispensing. Add five to eight drops
of the stain to the CenSlide tube
(Figure 3.1).
Figure 3.1