Iris Sample Processing StatSpin® CenSlide 2000 Urine Sediment Analysis System - Manual (Page 20)

Special Topics
OPERATOR’S MANUAL
CenSlide 2000 Urinalysis System Page 3 - 6
4. Mix the acetic acid into the CenSlide tube’s viewing area
by bending it five times, hand flicking it five times, and
then bending it five more times.
5. Place the CenSlide tube in the StakRak™ horizontally
and allow the urine sediment to settle for two minutes
prior to microscopic examination.
3.3 Microscope Objectives
3.3.1 Background
Examination of urine is normally done with a 10x objective for
an overview evaluation, followed by closer examination with a
40x objective.
Over the years, it has become standard to use a 1.0 mm
thick microscope slide, with a glass coverslip of 0.17 mm
thickness for the examination of urine under the microscope.
The optics in the objectives are ground to exacting tolerances
which take into account the refractive index of glass, which
determines how much light rays are bent by a specific
thickness.
With the advent of procedures such as tissue culture, where
the containers are usually of plastic and may be thicker than
a glass coverslip, it was found that the optics had to be
modified so they were able to focus through the increased
thickness and also deal with the refractive index problem of
the various plastics being used. These special objectives
were more costly and did not find their way into the world
of “routine” microscopes.
Note: Bending and hand flicking the viewing area causes an
increase in the number of cellular elements in the viewing area.
Due to this increase, quantitative analysis will not be valid as
cellular element levels will be falsely elevated.