Iris Sample Processing StatSpin® CenSlide 2000 Urine Sediment Analysis System - Manual (Page 19)

Special Topics
OPERATOR’S MANUAL
CenSlide 2000 Urinalysis System Page 3 - 5
3.1 Confirmatory Protein Assay (cont.)
3. After centrifugation, remove the CenSlide tube from the
centrifuge and decant the supernatant into a clean, clear
tube. Be very careful when decanting the supernatant not
to disturb the sediment at the bottom of the CenSlide tube.
4. Add an equal volume of sulfosalicylic acid (Exton’s
Reagent) to the decanted supernatant and mix.
5. Grade for cloudiness.
3.2 Differentiating Red from White Blood Cells
One of the most common methods of differentiating red blood
cells from white blood cells is by the addition of a few drops
of 2% acetic acid. The acetic acid will lyse only the red blood
cells leaving the white blood cells intact.
Red blood cells are effectively lysed in the CenSlide tube with
the addition of 6 drops of 2% acetic acid. However, because
acetic acid is a weak acid, basic urine specimens may
require a greater number of drops of 2% acetic acid in order
to lyse the red blood cells.
To lyse red blood cells using 2% acetic acid:
1. Process the CenSlide tube as you normally would
(see Sections 2.5 thru 2.7).
2. After the microscopic analysis has been performed,
uncap the CenSlide tube and decant the supernatant.
3. Add 6 drops of 2% acetic acid to the CenSlide tube.