1 procedure for measuring reducing power, Working procedures – Hach-Lange DR 5000 brewerie analysis, supplementary software LZV570 User Manual

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Working procedures

2. After the carbon dioxide has been expelled, pipette 10 ml beer

into a test tube with a glass stopper, then tilt the tube slightly
and add 0.25 ml 0.005M DPI solution.

3. Close the test tube immediately and invert it twice to mix the

contents, starting the stopwatch after the first inversion.

4. Immediately fill a 10 mm rectangular cuvette with the mixture.

60 sec after adding the reagent, measure the absorbance at
520 nm against a blank value solution (decarbonated beer
without added reagent).

Results
The results are expressed as the proportion of the DPI-amount
(in %) that was reduced by 10 ml beer in 60 sec.

Accuracy
V

cr

= ± 1%

Standard values
> 60 very good
50–60 good
45–50 satisfactory
< 45 poor

Literature
MEBAK Brautechnische Analysenmethoden
4th Edition 2002 Volume II, pp 104ff

2.3.1 Procedure for measuring reducing power

1. Prepare samples as described in the working procedure.

2. Select

STORED PROGRAMS

in the “Main Menu”. Select test

number

804

.

3. Insert blank value cuvette (see sample preparation) into the cell

compartment. Close the cell compartment. Touch

ZERO

.

4. Insert the sample cuvette containing the prepared sample into

the cell compartment and close the cell compartment. After 60
sec touch

READ

. The result is displayed.

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