Metrohm 754 Dialysis Unit - Manual (Page 23)

2 Installation
754 Dialysis Unit
20
2.3.7 Conditioning the dialysis system
Before effecting the first analysis, rinse the dialysis cell, the inserted di-
alysis membrane and all tubing connections with ultrapure water. Pro-
ceed as follows:
1 Settings on the 733 IC Separation Center
• Press the <FILL> key for injection valve A on the 733 IC
Separation Center. This switches injection valve A to the
"FILL" position.
• Press the <FILL> key for injection valve B on the 733 IC
Separation Center. This switches injection valve B to the
"FILL" position.
2 Starting up the 754 Dialysis Unit
• Immerse the two suction tubings 26 26 and 27 27 in the acceptor
solution (ultrapure degassed water).
• Set the <REMOTE> switch 22 on the 754 Dialysis Unit to
"OFF".
• Switch on the 754 Dialysis Unit with mains switch 13 13 and set
the <PUMP> switch 33 to "ON".
• Adjust the contact pressure for both tubing cartridges: Press
contact pressure lever 55 upwards until the solutions just start
to be drawn in. Then push the contact pressure lever up-
wards until it clicks again to achieve the optimum contact
pressure.
• Rinse the dialysis system with ultrapure water for approx.
10 min and check that the solutions flow steadily through the
two tubings into the waste bottle.
3 Conditioning the dialysis membrane
• Press the <INJECT> key for injection valve B on the 733 IC
Separation Center. This switches injection valve B to the
"INJECT" position.
• Rinse the dialysis system with ultrapure water for approx.
20 min. Check that the solutions flow steadily through the two
tubings into the waste bottle.
• Check all tubing from the storage vessels through the tubing
cartridges and the dialysis cell up to the waste containers for
any leaks. If liquid escapes anywhere, tighten or replace the
corresponding connection.
• If air bubbles remain in the dialysis cell, unscrew PEEK tubing
25 25 (Acceptor solution) and PTFE tubing 45 45 (sample) from
outlets 41 41 and 44 44 of the dialysis cell and wait until the air
bubbles disappear. Reconnect the tubings to the dialysis cell.