Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 10)

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Section 2
Instructions
2.1. General Considerations
Best results are obtained by using clean technique.
Any dust or dirt transferred to the surface of the gel
may appear in the fluorescence image as smudges or
speckles. Oriole
™ fluorescent gel stain is exceptionally
sensitive. Contaminant proteins such as keratin will
appear in the gel image if care is not taken to minimize
such contamination.
All glassware used should be cleaned with laboratory
glassware cleaner and rinsed with distilled or deionized
water. Use dust-free gloves and limit dust exposure by
keeping reagent vessels and gel trays covered as much
as possible. If gels are cast in the laboratory, the glass
plates used should be thoroughly cleaned with lint-free
laboratory wipes.
Oriole fluorescent gel stain is very sensitive, and less
protein can be visualized than what is possible using
a visible stain like Coomassie Blue. Sensitivity is of the