Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 23)

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Problem Possible Cause Remedy
Poor staining Reuse of the stain Reuse of Oriole stain is
sensitivity not recommended.
Use of Use only methanol and
nonrecommended provided diluent.
solvent for the 5 L kit
Fixing or washing Do not fix or wash gel prior
the gel prior to to staining.
staining
High or uneven Dirty equipment Make sure that the staining
background or staining trays trays and other equipment
staining have been thoroughly
cleaned with laboratory
glassware cleaner.
Too much time in Restrict time of
staining solution staining solution
treatment to 90–120 min.
Background resulting from
overstaining can be reduced
by washing the gel in water
for 30 min or more.
continued