Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 24)

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Problem Possible Cause Remedy
High or uneven Reagent impurities Use high quality reagents.
background when preparing stain
staining solution from the
5 L kit
Speckles or Particulate material Make sure that the
blotches in the from reagents, staining trays are
gel image staining tray, dust, thoroughly cleaned.
or gloves
Limit the time that the gels
and staining solution are
exposed to open air.
Use dust-free gloves and
handle gels only by
the edges.
Uneven staining Insufficient shaking Ensure that the gel
during staining is well agitated
during staining.
Gel shrinkage Some gel shrinkage The gel will reswell
occurs during staining following transfer to water.