Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 18)

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Section 3
Technical Information
3.1. Sensitivity of Staining — 1-D Gels
The dye in Oriole
™ stain is highly fluorescent and binds
tightly to proteins. Background staining is low, and the
limit of sensitivity is generally below 1 ng.
Fig. 2. Unadjusted image of a gel stained with Oriole stain.
A dilution series of Bio-Rad SDS-PAGE standards was run on a
4–20% Criterion Tris-HCl linear gradient gel, stained with Oriole
stain, and imaged using a Molecular Imager VersaDoc MP 4000
imaging system with image settings for SYPRO Ruby stain.
The resulting image file was not adjusted.