Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 11)

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same general order as silver stain or other fluorescent
protein stains. Oriole fluorescent gel stain has a wide
dynamic range, and variability in the amount of protein to
be visualized can be accommodated simply by varying
the exposure settings during imaging. As a general rule,
the maximum quantity of protein recommended for
visualization with Oriole fluorescent gel stain is 1–2 µg for
individual proteins and 10–20 µg for complex mixtures on
1-D gels. The limit of sensitivity for individual proteins is
1 ng or less.
Oriole fluorescent gel stain is moderately light sensitive.
If gels are left in stain for more than 90 min, the gel tray
should be covered with aluminum foil or an opaque lid.
Oriole fluorescent gel stain is intended only for staining
1-D and 2-D SDS-PAGE gels. Native gels and IEF gels
cannot be stained with Oriole stain. Oriole stain is not
recommended for staining protein blots.
Instructions given are for standard 1 mm thick SDS-PAGE
gels. Thicker gels may benefit from longer stain times and
larger volumes of solution.