Bio-Rad 2-D Electrophoresis Workflow User Manual
Page 20
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16
Fig. 4. Linearity of Oriole stain. A dilution series of carbonic
anhydrase was run on a Criterion gel, stained with Oriole stain,
and imaged on the Molecular Imager VersaDoc MP 4000 imaging
system. Fluorescence associated with the carbonic anhydrase
band was plotted following background subtraction.
Lo
g (
flu
or
es
ce
nc
e)
,
ar
b
itr
ar
y u
ni
ts
3.4. Dynamic Range
Oriole fluorescent gel stain has a dynamic range of up to
three orders of magnitude. This allows protein quantitation
in samples of varying concentration over a wide range of
relative abundance.
4.5
4
3.5
3
2.5
2
0
0.5
1
1.5
2
2.5
3
Log carbonic anhydrase, ng
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