Introduction and general information – Bio-Rad 2-D Electrophoresis Workflow User Manual

Page 5

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Section 1

Introduction and General Information

1.1. Introduction
Oriole

fluorescent gel stain is an easy to use, rapid, and

sensitive stain for visualization and quantitation of proteins
separated by SDS-PAGE. The product is available in
three configurations. The 200 ml and 1 L sizes are
provided ready to use. The product is also available as a
kit containing components to make 5 L of ready to use
staining solution.

The staining procedure is a simple one-step protocol
that can be completed in as little as 90 minutes.
Gels stained with Oriole fluorescent gel stain may
be visualized with a variety of different UV-based
fluorescence imaging systems.

Oriole fluorescent gel stain gives exceptional sensitivity
and dynamic range (see pages 14–16) and is compatible
with subsequent analysis by enzymatic digestion and
mass spectrometry. It is thus particularly well suited to
proteomics applications.

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