Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 15)

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2.4. Gel Imaging
Gels stained with Oriole stain are visualized using UV
light excitation. Bio-Rad Molecular Imager Gel Doc XR+,
Molecular Imager ChemiDoc XRS+, EXQuest spot cutter,
VersaDoc MP 4000, or VersaDoc MP 5000 systems are
recommended for imaging gels stained with Oriole stain.
If the imaging equipment has no preprogrammed imaging
function for Oriole fluorescent gel stain, the imaging
setting for SYPRO Ruby stain or ethidium bromide that
uses UV transillumination is recommended.
Any imaging system using UV light excitation may be
used to image Oriole fluorescent gel stain. Such imaging
systems almost always have midrange (300 nm, 306 nm,
or 312 nm) UV excitation and red or orange emission filters
as standard options for imaging ethidium bromide–
stained gels. The excitation and emission properties of
Oriole stain are very compatible with ethidium bromide,
therefore imager settings for ethidium bromide can be
used when imaging gels stained with Oriole stain.
Imaging systems capable of imaging gels stained with
Oriole fluorescent gel stain include the following: