Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 13)

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NOTE: Use only methanol in preparing staining
solution from the 5 L kit. The use of water, ethanol,
or other solvents will result in poor staining
performance.
Recommended Stain Volume
Volume of Stain
Gel Size Solution per Gel
Ready Gel ® or Mini-PROTEAN gel
(8.6 cm × 6.8 cm) 50 ml
Criterion gel (13.3 cm × 8.7 cm) 100 ml
PROTEAN II gel (16 cm × 16 cm or 16 cm x 20 cm) 250 ml
PROTEAN Plus gel (25.6 cm × 23 cm)
500 ml
2.3. Gel Staining
NOTE: Do not fix or wash gel prior to staining.
This will make staining less sensitive.
1. Place gel directly into a clean tray containing the
recommended volume of Oriole fluorescent gel stain.
Cover the tray, place on a rocker or shaker, and agitate
as vigorously as possible without splashing liquid or
damaging the gel.