Bio-Rad 2-D Electrophoresis Workflow - Manual (Page 22)

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Section 4
Troubleshooting
continued
Problem Possible Cause Remedy
Bands or spots No protein on gel Verify that there is actually
not visible protein on the gel by staining
with another method such as
Bio-Safe ™ Coomassie stain.
Malfunctioning Check instrument manual
imaging system for troubleshooting, or
contact the imaging
instrument manufacturer.
Poor staining Insufficient Stain sensitivity maximizes
sensitivity staining time after 90 min.
Dirty staining Make sure that the staining
trays trays and other equipment
have been thoroughly
cleaned with laboratory
glassware cleaner.
Insufficient Follow the recommendations
stain volume for stain volume appropriate
to the gel size (Section 2.2).