Bio-Rad Components for Older Model Spot Cutter - Manual (Page 3)

Table of Contents
Page
Section 1 Introduction ..................................................................................................1
1.1 General Description....................................................................................................1
1.2 Operation ....................................................................................................................1
1.3 Caution........................................................................................................................1
1.4
Moving The Spot Cutter.............................................................................................1
1.5 Computer Recommendations.....................................................................................1
1.6 Cutting Excision Sizes ...............................................................................................1
1.7 Power Setting .............................................................................................................1
Section 2 Getting Started .............................................................................................2
2.1 Preliminary Instructions .............................................................................................2
2.2 Connector Cables .......................................................................................................2
2.3 Software Setup ...........................................................................................................3
2.4 Initial System Set Up..................................................................................................3
2.5 Quick Guide to Spot Excision....................................................................................3
Section 3 How To Use Photo Enhancer and the Digital Camera ............................4
3.1 Turning on the Digital Camera ..................................................................................4
3.2 Selecting the DC120 Digital Access (TWAIN Acquire) Window ...........................5
3.3 Camera Functions.......................................................................................................5
3.4 Acquiring an Image....................................................................................................6
3.5 Saving an Image .........................................................................................................7
3.6 Erasing Picture Memory ............................................................................................7
Section 4 Selecting and Cutting Spots ........................................................................7
4.1 Opening an Image File Format ..................................................................................7
4.2 Using the Zoom Window...........................................................................................7
4.3 Selecting a Spot & MTP Location .............................................................................8
4.4 Deleting Spots ............................................................................................................8
4.5 Making Cuts (Excisions)............................................................................................9
4.6 Re-Cut Previous Spot .................................................................................................9
Section 5 Working Surface ........................................................................................10
5.1 Excision Mats ...........................................................................................................10
5.2 Cutting Surface.........................................................................................................11
5.3 Gel Sizer and Frames for 2-D Gels..........................................................................11
5.4 Microtiter Plate (MTP) Holder ................................................................................11
Section 6 Calibration ..................................................................................................12
6.1 Camera Alignment ...................................................................................................12
6.2 Calibration Definitions.............................................................................................12
6.3 Calibration Procedure...............................................................................................12
Section 7 The Gel Cutting System Menu Bar..........................................................16
7.1 File Menu..................................................................................................................16
7.2 View Menu ...............................................................................................................17
7.3 Cutting Menu............................................................................................................18
7.4 Operation Menu........................................................................................................19
7.5 Window Menu..........................................................................................................21
7.6 Help ..........................................................................................................................21