Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 12)

• Rinse off all of the detergent with warm water.
• Rinse with deionized water.
• Wipe the cleaned plate with a large lint free tissue to dry.
2. Inspect the plates carefully for pieces of detergent, dried polyacrylamide, or other particles.
Rewash if necessary.
3. Perform siliconizing under a fume hood, to reduce the hazard from breathing silanizing
reagent. Alternatively, several non-toxic, non-corrosive glass plate coating solutions are
commercially available. We recommend siliconizing or coating only the outer (long)
plate, so that when the plates are separated, the gel sticks to the IPC-bound glass plate.
• Use a glass Pasteur pipette to dispense 2 ml of the silanizing reagent onto the front
plate. Coat the plate completely and evenly by spreading the silanizing reagent on the
plate surface with a large lint free tissue, using a motion that travels from the top to the
bottom of the plate.
Caution: Do not siliconize the IPC plate unless hexane, heptane, or water is used as a
solvent in the silanizing reagent. Other organic solvents will craze or damage the IPC
plastic and weaken the adhesive bond.
• Never heat an IPC in an oven. Severe damage will result to the adhesive bond. Use
siliconizing compounds that react, or cure, at room temperature.
Note: If the gels will be fixed or stained, the IPC (short) plate should be siliconized or
coated, since its immersion into fixing or staining solutions is not recommended.
4. Prior to assembling the plates, apply a small amount of ethanol to each plate and rub to
dryness with a tissue. Using the same tissue, clean the spacers.
3.2 Cleaning Sequi-Gen GT Components
1. Rinse the universal base buffer chamber, stabilizer bar, combs, spacers and precision
caster base, gasket, syringe and tubing assembly with a mild detergent solution in warm
water. Use a soft-bristled brush or sponge to remove polyacrylamide gel pieces.
Note: Do not snag or break the electrode wire in the universal base while cleaning.
2. Rinse thoroughly with warm water and air dry.
Compatible Cleaning Agents for Polycarbonate Parts
Chemically compatible cleaners must be used to ensure long life of parts. These include:
• Aqueous solutions of soaps and mild detergents
• Organic solvents:
• Hexane
• Aliphatic hydrocarbons
• Alcohols
• Methanol
• Ethanol
• Isopropyl alcohol
• Dilute acids
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