Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 16)

2. Place the precision caster base on the bench with its open cavity facing up. Place the gray
precision caster gasket into the base. The cam pegs in the precision caster must be pulled
out to accommodate the apparatus.
Note: If the gasket is wet, remove any remaining water from the gasket by squeezing it
with a paper towel.
3. Place the bottom edge of the IPC assembly into the precision caster base with the bottom
edge of the assembly resting against the gray gasket of the precision caster base.
4. When the IPC assembly is seated in the caster base, use the cam pegs to connect the base
to the clamps (Figure 4.4).
• Push each cam peg into the corresponding hole on the clamp with the lever in the up
position. Slight downward pressure applied to the top of the IPC assembly may be
required to engage each cam peg.
Fig. 4.4. Attaching precision caster base to IPC assembly.
5. When both pegs are engaged, turn them evenly until moderate resistance is felt or the han-
dles of the cam pegs are perpendicular to the benchtop. This action causes the precision
caster base to fit tightly against the plate assembly.
• Lay the IPC assembly flat on the benchtop with the precision caster base facing toward you.
• Look through the injection port of the base. If the precision caster has been attached
properly, a space should be seen between the two green glass plates (Figure 4.5).
• If the space cannot be seen, loosen the caster base by rotating the cam pegs upward.
Adjust the caster base either up or down until the space between the green glass plates
can be seen and is in the middle of the injection port hole.
• While securing the precision caster base in place with one hand, turn the cam pegs
back to their original position to secure the base to the bottom of the IPC assembly.
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