Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 3)

Table of Contents
Page
Warranty Information ........................................................................Inside Front Cover
Section 1 General Information....................................................................................1
1.1 Introduction to Sequi-Gen GT DNA Electrophoresis Cell .......................................1
1.2 Specifications .............................................................................................................5
Section 2 Description of Major Parts .........................................................................6
2.1 Sequi-Gen GT Parts ...................................................................................................6
2.2 Gel Reagents and Electrophoresis Buffers ................................................................7
2.3 Electrical Path.............................................................................................................8
Section 3 Cleaning and Maintenance .........................................................................8
3.1 Cleaning and Siliconizing Plates................................................................................8
3.2 Cleaning Plastic Parts.................................................................................................9
Section 4 Operating Instruction................................................................................10
4.1 Before Assembly ......................................................................................................10
4.2 Assembling the Glass Plate Sandwich.....................................................................10
4.3 Casting the Gel .........................................................................................................12
4.4 Preparing for Operations ..........................................................................................17
4.5 Loading the Gel ........................................................................................................18
4.6 Gel Electrophoresis ..................................................................................................19
4.7 Disassembly..............................................................................................................20
Section 5 Troubleshooting Guide ..............................................................................22
5.1 Operational Troubleshooter .....................................................................................22
5.2 DNA Sequencing Artifacts ......................................................................................23
Section 6 Equipment and Accessories ......................................................................26
6.1 Sequi-Gen GT Nucleic Acid Electrophoresis Cells and Accessories .....................26
6.2 Electrophoresis Reagents .........................................................................................30
6.3 Power Supplies and Slab Gel Dryers.......................................................................31
6.4 DNA Template Purification, Sequencing, and Cloning Products...........................31
6.5 Liquid Handling .......................................................................................................32
Section 7 Appendix A- Applications .........................................................................32
7.1 DNA Sequencing Checklist .....................................................................................32
7.2 Standard Gel Protocol ..............................................................................................33
7.3 Gel Drying Autoradiography ...................................................................................34
7.4 Applications for Sequi-Gen GT Nucleic Acid Electrophoresis Cell ......................34
7.5 Suggested Reading ...................................................................................................35