Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 5)

Bio-Rad is not responsible for any injury or damage caused by the use of this instrument
for purposes other than those for which it is intended, or by modifications to the instrument
not performed by Bio-Rad or an authorized agent.
Power to the Sequi-Gen GT cell is supplied by an external DC power supply. This power
supply must be ground isolated in such a way that the DC voltage output floats with respect
to ground. The recommended power supply for this apparatus is the PowerPac 3000 power
supply. The maximum specified operating parameters for the Sequi-Gen GT cell are:
Maximum operating voltage – 3,000 VDC
Maximum operating power – 100 Watts
Electrical current to the Sequi-Gen GT cell enters the unit through the top and bottom
safety covers, providing a safety interlock to the user. Current flow to the cell is broken when
either safety cover is removed. Do not attempt to circumvent this safety interlock. Always
turn the power supply off while working with the sequencing cell when the safety covers are
not connected.
No user-serviceable parts are contained in this apparatus. To insure electrical safety, do
not attempt to service this apparatus.
Caution — Arcing
Arcing within an electrophoresis cell is represented by sparks, smoke, or charred surfaces
created when an electrical short has developed. Arcing can occur if the buffer level drops
below the recommended height, if there is buffer leakage, or if loose electrical connections
exists. If arcing is detected during electrophoresis, immediately remove the source of
electrical current (i.e., turn off the power supply).
Always use a power supply that is capable of detecting electrical conditions that may
cause accidental electrical shock or damage to the apparatus. The PowerPac 3000 power
supply contains safety features such as arc, no load, overload, rapid change in resistance, and
ground leak detection capabilities, that will reduce the chance of accidental electrical shock
and damage to the electrophoresis cell.
Before every use, inspect all plastic parts, glass plates, all electrical cables, jacks, and
receptors for loose connections, cracks, charring, or corrosion. Do not use any part that is
cracked, chipped, charred, or corroded. These parts may cause arcing. Contact your Bio-Rad
representative before using a part that may cause buffer leaking or arcing.
Warning:
Never allow the gel to exceed 60 ˚C. Excessive heat may crack the plates or cause the
silicon bond of the IPC to deteriorate.
Make sure that the upper and lower buffer chambers are filled with buffer during
electrophoresis. Do not allow the buffer level to drop below the level of the short glass plate
of the upper buffer chamber or below the bottom of the IPC assembly in the lower buffer
chamber at any time.
Certain solvents and cleaning agents should be avoided with this unit. Refer to Section 3.2
for compatible solvents, reagents and cleaning agents.
Definition of Symbols
Caution, risk of electrical shock Caution (refer to accompanying documents)
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