Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 35)

6.5 Liquid Handling
Catalog
Number
Product Description
223-9911 Seque/Pro Capillary Tips , 200/Box
223-9912 Seque/Pro Capillary Tips , 200/Box, autoclaved
223-9314 MTP-39 Pipet Tips , 960/Box
223-9319 MTP-39-S Pipet Tips , 960/Box, Sterilized
211-2001 Xcluda Aerosol Barrier Pipet Tips , 0.5–10 µl, 960/Box, Sterilized
211-2006 Xcluda Aerosol Barrier Pipet Tips , 5–20 µl, 960/Box, Sterilized
211-2016 Xcluda Aerosol Barrier Pipet Tips , 20–200µl, 960/Box, Sterilized
223-9480 EZ Micro Test Tube , 1.5 ml, 500/Box
223-9503 EZ Micro Test Tube , 0.5 ml, 500/Box
Section 7
Appendix
7.1 DNA Sequencing Checklist
For DNA sequencing, you will need the following buffers, reagents, and equipment:
1. DNA sequencing samples, suitably labeled. (see Section 6.4)
2. 10x TBE buffer: 108 g Tris base, 55 g boric acid, 9.3 g Na
2
EDTA•H
2
O, in 1 liter
deionized H
2
O, autoclave. The pH of this solution should be 8.3, without adjustment
(see Section 6.2).
3. Acrylamide stock solution, 30%:
A. For low percent gels (4%-10%):
Prepare a 30% stock solution, 19:1 Acrylamide/Bis:
28.5 g Acrylamide
1.5 g Bis-Acrylamide
30.0 g Total up to 100 ml deionized H
2
O
(see Section 6.2)
B. For medium percent gels (8%-16%):
Prepare a 30% stock solution, 29:1 Acrylamide/Bis:
29.0 g Acrylamide
1.0 g Bis-Acrylamide
30.0 g Total up to 100 ml deionized H
2
O
(see Section 6.2)
C. For high percent gels (12%-20%):
Prepare a 40% stock solution, 37.5:1 Acrylamide/Bis:
38.96 g Acrylamide
1.04 g Bis-Acrylamide
40.0 g Total up to 100 ml deionized H
2
O
(see Section 6.2)
4. TEMED (see section 6.2)
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