Bio-Rad Sequi-Gen GT Sequencing Cell - Manual (Page 6)

System Components
Each Sequi-Gen GT system comes with the components listed in Table 1.1. Check your
unit to be sure all items are present. Note any damage to the unit which may have occurred
during shipping. Notify Bio-Rad Laboratories if any items are missing or damaged.
Table 1.1. Sequi-Gen GT System Components
Item Quantity
GT Universal Base 1
Stabilizer Bar 1
GT Safety Covers 1 1
GT IPC, with bonded inner (short) glass plate 2 1
Outer (long) Glass Plate 2 1
GT Clamp Set (left and right clamp) 3 1
Precision Caster Base 1 1
Precision Caster Gasket 1 1
Precision Caster Syringe 4 1
Precision Caster Tubing, 60 cm 1
Precision Caster Luer Tapers 4
IPC Drain Port/Tubing Connector 1
Gel Temperature Indicator 1
Vinyl Spacers, 0.4 mm thick 3 2
Vinyl Sharkstooth Comb, 0.4 mm thick 5 1
Leveling Bubble 1
Instruction Manual 1
1 Parts come in 21 cm or 38 cm widths
2 GT IPC and Glass Plates are 21 x 40, 21 x 50, 38 x 30 cm or 38 x 50 cm sizes
3 GT Clamp Sets and Vinyl Spacers are either 30 cm, 40 cm or 50 cm lengths
4 Syringe sizes are 60 cc for 21 cm systems and 140 cc for 38 cm systems
5 Vinyl Sharkstooth combs are 24 well (25 teeth) for 21 cm units or 49 well (50 teeth) for 38 cm units
See Section 6 for information on accessories and replacement parts.
General Description
The Sequi-Gen GT DNA sequencing cell uses several innovative design features that are
especially useful for DNA/RNA sequencing or other nucleic acid separation applications.
Sequi-Gen GT DNA sequencing cell features and benefits include:
Features Benefits
Unique, horizontal, syringe injected gel Easy gel casting without tape, grease, and
casting method acrylamide spills and waste
Upper buffer chamber heat Provides uniform gel temperature that
distribution system prevents smiling
Permanently sealed upper buffer No gaskets or grease needed to provide leak-
chamber free electrophoresis
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