Bio-Rad Model 550, Ultramark, and Benchmark Microplate Reader Accessories - Manual (Page 16)

4.2 Quick Guide—Reading a plate:
1. Open the rear compartment and verify that the correct interference filters are properly
installed in the filter wheel. See Section 5.1 for instructions on changing the interference
filters. Close the rear cover.
2. Turn on the instrument. Allow one minute for the self diagnosis. Wait three minutes
before reading a plate.
While the instrument is warming up:
3. Enter the SET ANALYSIS mode (Section 4.3) to:
a. Choose single or dual wavelenth measurement
b. Choose the measurement and reference filters
c. Choose whether or not to mix
4. Enter the SET REPORT TYPES mode (Section 4.3) to choose the type(s) of reports to be
generated.
5. All reports other than the Raw report require blanks. Enter the SET BLANKS mode
(Section 4.3) to indicate the position of blanks.
6. The Matrix and Limit reports require assignment of upper and lower limits. If either of
these reports is requested, enter the SET LIMITS mode (Section 4.3) to set the upper and
lower limits.
7. The Cutoff report requires definition of the cutoff point. If the Cutoff report is requested,
enter the SET CUTOFF mode (Section 4.3) to define the cutoff point.
8. The Concentration report requires that standard concentrations and locations be defined.
If the Concentration report is requested, enter the SET CONC. mode (Section 4.3) to
define the standards.
After warm-up is complete:
9. Press MODE until the display reads
or
The bottom line of the display will read Single #x or Dual #y, #z, depending on the choice
made under the SET ANALYSIS mode.
10. Place the microplate in the reading chamber, slide the reading chamber door closed and
press START.
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PLATE READING
Single #x
PLATE READING
Dual #y, #z