Bio-Rad Model 550, Ultramark, and Benchmark Microplate Reader Accessories - Manual (Page 37)

8. <command>: “RPLATE” - read plate; arguments <mix>, <wp1>,<wp2>
<cmd args>: mix - Mixing time before reading (in seconds) ; ASCII encoded
decimal (0-9)
wp1 - Measurement filter position; ASCII encoded decimal (1-4)
wp2 - Reference filter position; ASCII encoded decimal (1-4)
Optional; only present for Dual wavelength reading
<response>: “ERE” <space><error code><space><abs. data><cr>
9. <command>: “RTPLATE” - retransmit the last plate read; no arguments
<response>: “ERE” <space><error code><space><abs. data><cr>
Absorbance Data
<abs. data>: <header><filter info>< Mes.data><cr> or <header><filter info>
<Mes. data><cr><Ref.data><cr> Ref. data present for dual wavelength readings.
<header>: “BIO-RAD MODEL 550 READER”<cr>
<filter info>: “Mes. filter:” <wp1><cr>
“Ref. filter:” <wp2><cr> - Present for Dual wavelength reading
<Mes. data>: “ . begin” <cr><8 rows of 12 wells of abs. values><checksum><cr>
“.end” <cr>
Measurement wavelength absorbance values are in ASCII encoded
floating point format. Each absorbance value is separated by a space.
Row1=wells (A1-A12), Row2=wells (B1-B12), .... Row8=wells (H1-
H12).
<Ref. data>: “ . begin” <cr><8 rows of 12 wells of abs. values><checksum><cr>
“.end” <cr>
Reference wavelength absorbance values are in ASCII encoded
floating point format. Each absorbance value is seperated by a space.
Row1=wells (A1-A12), ROW2=wells (B1-B12, ....Row8=wells (H1-
H12)
<checksum>: The checksum is a two byte sum in ASCII encoded decimal (0-255)
of all the ASCII characters transmitted in the measurement or
reference data block. The checksum does not include the character
string “.begin”<cr> in the checksum calculations.
The following is an example of both <Mes. data> and <Ref. data>:
“. begin”<cr>
“ 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 0.112”<cr>
“ 0.201 0.202 0.203 0.204 0.205 0.206 0.207 0.208 0.209 0.210 0.211 0.212”<cr>
“ 0.301 0.302 0.303 0.304 0.305 0.306 0.307 0.308 0.309 0.310 0.311 0.312”<cr>
“ 0.401 0.402 0.403 0.404 0.405 0.406 0.407 0.408 0.409 0.410 0.411 0.412”<cr>
“ 0.501 0.502 0.503 0.504 0.505 0.506 0.507 0.508 0.509 0.510 0.511 0.512”<cr>
“ 0.601 0.602 0.603 0.604 0.605 0.606 0.607 0.608 0.609 0.610 0.611 0.612”<cr>
“ 0.701 0.702 0.703 0.704 0.705 0.706 0.707 0.708 0.709 0.710 0.711 0.712”<cr>
“ 0.801 0.802 0.803 0.804 0.805 0.806 0.807 0.808 0.809 0.810 0.811 0.812”<cr>
<checksum><cr>
“ . end”<cr>
Note: Absorbance values greater then 3.000 shall be transmitted as an asterisk.
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