Bio-Rad ZOE™ Fluorescent Cell Imager - Manual (Page 16)

ZOE Fluorescent Cell Imager Instruction Manual 8 |
Live Mode
The Live function is used for viewing samples illuminated in one of the four channels (brightfield
or one of the three fluorescent channels) and for acquiring cell images.
Motorized Stage and Field of View
The ZOE Cell Imager’s motorized stage, with up to 6 mm of travel, enables rapid visualization
of a large sample area. Direction and speed of the stage movement are controlled through the
touch screen, and display magnification can be instantly increased using the pinch-to-zoom
gesture. X and Y coordinates (Figure 4) are updated as the stage moves. Use the Center icon
(Figure 4) to return the stage to the Home position (X,Y: 0,0).
Using the Brightfield Channel
A control panel located on the left side of the screen contains controls for switching on and off
light sources for the individual imaging channels (Figure 4). For improved image contrast, a ring
of green LEDs is the light source in the brightfield channel. ZOE uses a monochrome camera
and the resulting brightfield images are black and white.
Tap the illumination control icon (Figure 3) to turn the LED light source on, tap again to
turn it off.
Note: Photobleaching due to this green light source is comparable to that of a regular white
light. The wavelength used does not cause the red fluorophore to fluoresce or photobleach.
Fig. 3. Brightfield channel light source on/off.
Fig. 4. User controls available in Live mode.
Settings
Swipe the screen to move
stage in desired direction
Light
sources
X,Y axes:
2-D vertical,
horizontal
stage
positions
Z axis: Focus
position
Precapture
adjustments
Tap to
change
functional
mode
Tap to
capture an
image
Tap to center
motorized
stage
Pinch-to-zoom and change
magnification