Bio-Rad ZOE™ Fluorescent Cell Imager - Manual (Page 19)

| 11 ZOE Fluorescent Cell Imager Instruction Manual | 11
Optimizing Imaging Parameters for Fluorescence Imaging
Use the Edit menu to adjust these precapture parameters (Figure 7): Gain, LED Intensity, and
Contrast values; Exposure time (msec); and quadrant illumination. Drag the slider bar to adjust a
parameter. Tap the – and + controls at either end of each slider bar for fine adjustments.
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When searching for sample — increase the Gain value for brighter signal; reduce the
Exposure time to obtain a faster frame rate
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Once the sample is identified and ready to be captured — lower the Gain value
to reduce the background noise; increase the Exposure time to regain signal intensity
as needed
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To further increase brightness — start by increasing LED intensity value; follow by
increasing Exposure time if needed
Fig. 8. Samples pseudo-colored in the appropriate emission color in Live mode. A, BPAE cells, mitochondria stained with
MitoTracker red CMXRos; B, BPAE cells, F-actin stained with Alexa Fluor 488 phalloidin.
A B
Fig. 7. Edit controls in fluorescent channels.
Gain: 1
Apply Cancel
Exposure: 50
LED Intensity: 50
Contrast: 50