Bio-Rad ZOE™ Fluorescent Cell Imager - Manual (Page 17)

ZOE Fluorescent Cell Imager Instruction Manual | 9
Focus position indicator on the left side of the touch screen shows the Z axis position number.
The number is updated during focusing (Figure 4). Knowing the approximate Z number for the
tissue culture vessels (Table 2) you use can speed up focusing.
Table 2. Z axis position range of tissue culture vessels.
Vessel Type
Z Axis Position
Microscope slide 720 (± 100)
6-well plate 2,000 (± 100)
12-well plate 2,100 (± 100)
24-well plate 1,900 (± 100)
48-well plate 2,000 (± 100)
96-well plate 3,500 (± 200)
T25 flask 1,800 (± 200)
T75 flask 2,500 (± 200)
T225 tissue culture flask 3,200 (± 200)
Small petri dish 1,200 (± 200)
Large petri dish 1,100 (± 200)
Optimizing Imaging Parameters in Brightfield Channel
Use the Edit menu to adjust these precapture parameters (Figure 5): Gain, LED Intensity, and
Contrast values; Exposure time (msec); and quadrant illumination. Drag the slider bar to adjust a
parameter. Tap the – and + controls at either end of each slider bar for fine adjustments.
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When searching for sample — increase Gain value for brighter signal; reduce Exposure
time for faster frame rate
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Once the sample is identified and ready to be captured — lower Gain value to reduce
background noise; increase Exposure time to regain signal intensity
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To further increase brightness — start by increasing LED Intensity value; follow by
increasing Exposure time if needed
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To reduce nonspecific background signal — increase Contrast value
Fig. 5. Edit controls in the brightfield channel.