Bio-Rad ZOE™ Fluorescent Cell Imager - Manual (Page 9)

ZOE Fluorescent Cell Imager Instruction Manual | 1
Introduction
1
The ZOE Fluorescent Cell Imager is an inverted imaging system with brightfield and three
fluorescent channels (emitting in blue, green, and red color), suitable for routine cell culture
and imaging applications. All channels are fully integrated and optimized for most commonly
used fluorescent proteins and dyes; no calibration or installation is needed. It is a stand-alone
instrument and a PC is not needed to operate it.
The intuitive touch screen allows researchers to visualize their samples and capture cell
images with the integrated digital complementary metal-oxide semiconductor (CMOS) camera.
Images stored in the internal memory can be edited, overlaid into multicolor merges, and/or
exported to a USB key using one of the two USB ports. The integrated light shield removes the
need for a dark room and allows visualization of fluorescence in ambient light on the bench,
where researchers work with cells. The screen viewing angle can be adjusted easily and
optimized to a user’s body height.
Each LED light source provides thousands of hours of reliable illumination that are instantly
ready after power on. They provide cool, even, and continuous illumination; their brightness
can be adjusted by the user to reduce sample photobleaching.
Focusing is conducted manually using coaxial coarse/fine focus knobs located on the instrument
base in an ergonomically deduced position, thus minimizing the strain on the user’s hands.
The ZOE Cell Imager’s 20X lens is mounted in a proprietary manner that results in a large field
of view, one that is approximately equivalent to that of a 4X objective lens (0.70 mm
2 ). Using
the pinch-to-zoom gesture, researchers can instantly change magnification while retaining
resolution of 1 µm.
Thanks to the large field of view and a motorized stage (up to 6 mm of travel), a large sample
area can be visualized rapidly. This attribute is useful when assessing transfection efficiency
or cell confluency. The direction and speed of the stage’s movement are controlled through
the touch screen.