Bio-Rad Model 2110 Fraction Collector - Manual (Page 15)

5.2 Carousel Installation
The Model 2110 Fraction Collector fills tubes from the outside of the
carousel inward. Load as many tubes as desired into the carousel, starting
at the outside end of the spiral and moving inward. The carousel need not
be fully loaded, since any eluant flowing after the last tube will drain
through the carousel into the well in the case and out the central drain tube
located at the rear of the instrument.
1. Swing the drop arm to the waste (rear) position by gently lifting and
rotating (Figure 5.1a).
2. Hold the carousel so that the notch in its outline is toward the rear of
the instrument.
3. Place the carousel on the pivot shaft, holding it about 1 ¼
2
" above its
seated position.
4. Push the carousel and the pivot shaft toward the rear of the
instrument, settling it down over the drive gear so that the gear is
trapped near the start of the spiral.
5. Holding the gear teeth free from engagement, turn the carousel coun-
terclockwise until it stops against the gear (Figure 5.1b). Release the
carousel and it will spring into engagement on the drive gear.
Engagement may be confirmed by trying to move the carousel gently
(Figure 5.1c).
6. Check the alignment with the two positioning guides located on the
top front of the instrument case. The number corresponds to the first
tube that will be filled.
Swing the drop arm into the collect (front) position. Make sure the
drive gear is fully seated into the grooved underside of the carousel. This
automatically aligns the drop former over the first tube.
5.3 Collection Tube Installation
Load the carousel with up to eighty 13 x 100 mm test tubes. You may
find a tube which fits too tightly into the carousel. It is best to discard
these rather than risk breaking the tube upon installation. Tubes 39
through 53 are raised above tubes 1-38 to make tube removal easier.
To locate an individual tube in a carousel, find its number on the
angled flange. The tube is in line with the number and the center of the
carousel. The top row of numbers corresponds to the outermost row of
tubes, the middle row corresponds to the next row of tubes, and the bot-
tom row to the innermost row of tubes. To divert a large amount of eluant
to waste at the beginning of a run, you can simply remove the first
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