Bio-Rad Nuvia™ IMAC Resin - Manual (Page 16)

12 Nuvia IMAC Ni-Charged Resin
Resin Preparation
Nuvia IMAC Ni-charged resins come in a 20% ethanol solution,
supplied for resin storage. The column can be slurry packed in
this solution; simply resuspend the resin in the ethanol solution
provided. Before applying sample, ensure that all ethanol is
removed during the equilibration step.
Method
1. Eliminate air from the column dead spaces. Attach the inlet
of a peristaltic or other pump to the outlet of the MT column.
Fill the column with distilled water to about 10% of its volume.
Flush end pieces with distilled water to ensure that the bottom
of the bed support is fully saturated and free of air bubbles.
Allow a few centimeters of distilled water to remain when
closing the outlet valve.
2. Suspend the 50% v/v slurry by gently swirling or stirring with a
glass or plastic rod.
3. Carefully transfer about a third of the slurry down the side into
the column using a glass or plastic rod to avoid introducing air
bubbles.
4. Start the pump at a low flow rate (eg. 0.5 ml/min). The resin
will begin to pack in the column. As the liquid level in the
column drops, continue to transfer the rest of the slurry until
the packed bed reaches 1 cm from the top. Stop or slow the
pump flow rate as necessary.
Gently add more distilled water down the side of the column
to make sure the liquid does not fall below the resin level.
Continue adding distilled water until the bed seems to have
stabilized. Then gently fill the column to the top with distilled
water.
5. Stop the pump and prepare to insert the top adapter.
6. Insert the top flow adaptor. Use care to avoid introducing any
air bubbles. Insert the adaptor into the column at an angle.
Make sure the exit tubing is open so that distilled water can
flow out the top adaptor along with any air.