Bio-Rad Nuvia™ IMAC Resin - Manual (Page 6)

2 Nuvia IMAC Ni-Charged Resin
Section 2
Product Information
Nuvia ™ IMAC Resins and UNOsphere ™ Technology
Nuvia IMAC resin, a unique affinity support, is based on Bio-Rad’s
innovative UNOsphere beads, which use proprietary polymerization
and derivatization technologies.* The UNOsphere technology
enables the polymeric high-capacity IMAC resin to exhibit excellent
flow properties without compromising protein binding, recovery, or
purity.
Nuvia IMAC uses NTA as its functional ligand. The tertiary amine
and carboxylic acid side chains of NTA serve as the chelating
ligands for divalent metal ions. The structure offers selective
binding of recombinant histidine-tagged proteins when this resin is
charged with Ni
2+ or other transition metals. As a result, the desired
proteins can often be purified close to homogeneity in a single
step.
Structural characteristics such as the polymeric nature, optimized
ligand density, and open-pore structure of Nuvia IMAC beads
result in superb mechanical strength with high stringency, low
nonspecific effects, and the ability to provide separations at fast
flow rates. These unique features of the UNOsphere base matrix
lend a number of performance benefits to the Nuvia IMAC resin.
Nuvia IMAC is also stable across a wide pH range (2–14) and
is compatible with most reagents commonly used in protein
purifications, such as denaturants, detergents, and reducing
agents. It is amenable to separations under native or denaturing
conditions using liquid chromatography instrumentation, gravity
flow columns, or sample-preparation spin columns.
Note: UNOsphere media, from which Nuvia IMAC is derived,
was designed to achieve the highest productivity (grams of
drug or target per operational hour per liter of support) possible.
UNOsphere media may be run at the highest rates and loading
capacities and will stay within the pressure limits of the column and
chromatography system.
* U.S. patent 6,423,666.