Bio-Rad Nuvia™ IMAC Resin - Manual (Page 7)

Nuvia IMAC Ni-Charged Resin 3
Under optimized conditions, the binding capacity for 6x histidine-
tagged proteins is >40 mg/ml resin (see Table 1). The product is
a 50% (v/v) slurry of resin, which is suspended in a 20% ethanol
solution. Nuvia IMAC resin is amenable to process and laboratory-
scale use and is available precharged with Ni
2+ in bottles as well
as prepacked into columns. Table 1 lists key characteristics of the
resin, while Table 2 lists a variety of compounds compatible with
Nuvia IMAC support.
Chemical Interactions
Nuvia IMAC resin is composed of NTA groups coupled to
a UNOsphere base matrix via a proprietary polymerization
derivatization technology. It is well-suited to recombinant histidine-
tagged purifications and results in high binding capacity and
specificity for the target molecule.
Although the most commonly used metal ion for histidine-tagged
purifications is Ni
2+ , other metals may be used to increase efficacy
of purification. Therefore, choosing another type of immobilized
metal ion can change the selectivity of an IMAC resin.
Fig. 1. Partial structure of Nuvia Ni-charged IMAC resin. Image illustrates UNOsphere base
bead with coupled NTA functional ligand.