Bio-Rad Nuvia™ IMAC Resin - Manual (Page 17)

Nuvia IMAC Ni-Charged Resin 13
7. Adjust the adaptor to sit directly on top of the resin bed.
Open the column outlet and pump distilled buffer through the
column at a) a packing flow rate of ~400–600 cm/hr for 5 to
10 min, or b) the maximum pressure allowed by the column
hardware and resin. The resin bed will compress while packing
at high flow rates. Mark the compression level with a pen.
Stop the flow. At this point, the resin bed height may readjust
and rise. If this happens, adjust the flow adaptor to compress
the bed another 0.1–0.5 cm past the level marked with the
pen.
8. Reconnect the pump and equilibrate. Pass eluent (distilled
water) through the column at the packing flow rate. During
equilibration, the bed may compress even further. When a
constant bed height is reached, mark the compression level at
this flow rate. Again, adjust the adaptor to compress the bed
an additional 0.1–0.5 cm past the level marked.
Note: Chromatographic steps during purification should not be
run at greater than 75% of the packing flow rate.