Bio-Rad Nuvia™ IMAC Resin User Manual
Page 4
Section 8 Medium-Pressure Column Purification — Using
an Imidazole Gradient to Determine Optimal
Purification of Histidine-Tagged Proteins ........... 21
Materials ................................................................. 21
Method ................................................................... 22
Section 9 Sample Preparation–Sized Spin-Column
Purification of Histidine-Tagged Proteins ........... 25
Materials .................................................................25
Method ...................................................................26
Section 10 Regenerating, Cleaning, Sanitizing,
and Storage ..........................................................28
Regenerating the Medium .......................................28
Cleaning in Place ...................................................28
Sanitization..............................................................30
Storage .................................................................. 30
Section 11 Troubleshooting Guide ......................................... 31
Section 12 Ordering Information ........................................... 34
Section 13 References ........................................................... 34
Section 14 Legal Notices ...................................................... 34