Bio-Rad Nuvia™ IMAC Resin - Manual (Page 34)

30 Nuvia IMAC Ni-Charged Resin
Sanitization
Sanitization inactivates microorganisms and prevents buildup of
endotoxins. For optimal results, the column should be run at
300 cm/hr.
The column may be washed with the following solution:
• 1 M NaOH
Rinse solution from column with 3–5 column volumes of distilled
water. Re-equilibrate the column with 3–5 column volumes of
binding buffer.
Storage
Nuvia IMAC resin is stable at room temperature across a broad
pH range (2–14). The medium may also be stored in either of the
following solutions:
• 2% benzyl alcohol
• 20% ethanol