Bio-Rad Profinity IMAC Resins - Manual (Page 12)

FPLC Systems
The luer to M6 adaptor fittings kit* provides fittings
necessary to connect the cartridge to the M6 fittings
found on FPLC or related systems.
Section 5
Buffers and Methods
IMAC methods can be run using either native or
denaturing purification protocols. Under native
conditions, proteins are purified using buffers that
help retain the natural folded structure of the target
protein. Under denaturing conditions, strong
chaotropes (typically 6 M urea or guanidine) are
added to the buffers, allowing target proteins to be
purified in their unfolded states. The recommended
buffer compositions and formulations are provided
in the following two tables:
*Fittings kit ordering information can be found within
Section 12 of this manual.
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