Bio-Rad Profinity IMAC Resins User Manual

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Table of Contents

Section 1

...

Introduction ........................................1

Section 2

Product Information............................2

Section 3

Connection to Low-Pressure
Chromatography Systems ..................6

Section 4

Connection to Medium and High-
Pressure Chromatography Systems ...8

Section 5

...

Buffers and Methods ..........................9

Section 6

Quick Solubility Screening
Protocols ..........................................11

Section 7

Preparation of E. coli Lysates ...........15

Section 8

Preparing a Cartridge, and
Subsequent Purification....................17

Section 9

Scaling Up........................................21

Section 10 Regenerating, Cleaning,

Sanitizing, and Storage.....................22

Section 11 Troubleshooting Guide......................25

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