Bio-Rad Profinity IMAC Resins - Manual (Page 7)

Table 2. Profinity IMAC Resin Specifications
Functional ligand IDA
Base bead UNOsphere
Particle size range 45–90 µm
Mean particle size 60 µm
Metal ion capacity 12–30 µmol Cu 2+ /ml
Dynamic binding capacity* ≥15 mg/ml
Recommended linear flow rate 480 cm/hr
Maximum operating pressure 45 psi
Chemical compatibility See table
Storage 4°C to ambient temperature
Shelf life in 20% EtOH >1 year at ambient temperature
Operational temperature 4–40°C
* Q10% determination of 1.8 mg/ml (His)6-tagged pure protein (32 kD).
Note: Dynamic binding capacity will vary from protein to protein.
Profinity IMAC cartridges are compatible with aqueous
buffers most commonly used with IMAC purification
techniques. For a complete list of chemical
compatibilities, refer to the Profinity IMAC
instruction manual, online at http://www.bio-
rad.com/cmc_upload/Literature/164111/10001677
B.PDF.
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