Bio-Rad Profinity IMAC Resins - Manual (Page 8)

Table 3. Buffer and Chemical Compatibilities for
Profinity IMAC cartridges
Reagent Stability
Buffer Reagents
Tris 50 mM
HEPES 50 mM
MOPS 50 mM
Sodium or potassium phosphate 50 mM
Chelating Agents
EDTA, EGTA 0.1 mM
Sulfhydryl Reagents
β−Mercaptoethanol 30 mM
DTT 5 mM
TCEP 10 mM
Detergents
Nonionic detergents (Triton, Tween, NP-40) 5%
Cationic detergents (CTAB) 1% (care must be taken to
avoid protein precipitation)
Zwitterionic detergents (CHAPS, CHAPSO) 5%
Anionic detergents (SDS, Sarkosyl) 1%
Denaturing Agents
Guanidine-HCl 6 M
Urea 8 M
Other Additives
NaCl 2 M (at least 300 mM NaCl
should be included in buffers)
MgCl
2
100 mM (HEPES or Tris
should be used to prevent
precipitation)
CaCl
2
10 mM (HEPES or Tris
should be used to prevent
precipitation)
Glycerol 20%
Ethanol 20%
Imidazole
25 mM in wash buffer
500 mM for elution
Citrate 80 mM
* Profinity IMAC binding capacities are unaffected with typical reagents used for
His-tagged protein purification, up to the concentrations given.
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