Bio-Rad Profinity IMAC Resins - Manual (Page 26)

For the 1 ml cartridges, run the cleaning protocol at
2 ml/min. It is recommended that the 5 ml cartridge
cleaning protocol be run at 5 ml/min.
High Salt/Acid Cleaning
1. Rinse the cartridge with 2 CV water at 2 ml/min.
2. Wash the cartridge with 5 CV 500 mM NaCl,
50 mM Tris, pH 8.0 at 2 ml/min.
3. Wash the cartridge with 5 CV 500 mM NaCl,
100 mM NaOAc, pH 4.5 at 2 ml/min.
4. Rinse the cartridge with 2 CV water at 2 ml/min.
5. Store the cartridge in 20% ETOH at 4°C.
Chaotrope Cleaning
1. Rinse the cartridge with 2 CV water at 2 ml/min.
2. Wash the cartridge with 5 CV 6 M guanidine
HCl at 2 ml/min.
3. Rinse the cartridge with 2 CV water at 2 ml/min.
4. Store the cartridge in 20% ETOH at 4°C.
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