Bio-Rad Profinity IMAC Resins - Manual (Page 4)

Section 1
Introduction
Bio-Scale Mini IMAC cartridges are convenient,
disposable, prepacked low-pressure chromatographic
cartridges. Bio-Scale Mini cartridges offer both
increased run-to-run uniformity and high purity of
protein through a patent pending column design
and novel resin technology. Compatible with aqueous
buffers most commonly used for protein purification
the Bio-Scale Mini cartridges offer improved
performance for your protein separation needs.
Immobilized metal affinity chromatography (IMAC) is an
excellent chromatography technique for purification of
His-tagged proteins. The principle of IMAC is based
on the affinity histidine has for metal ions. Side chains
on the iminodiacetic acid (IDA) functional ligand
selectively bind recombinant His-tagged proteins when
the resin is charged with Ni
2+ or other metals. The
advantage of this technique is that proteins can often
be purified close to homogeneity in a single step.
Bio-Scale Mini IMAC cartridges are packed with
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