Bio-Rad Bio-Scale™ Mini Affi-Gel® Blue Cartridges - Manual (Page 20)

same instructions. After connecting the cartridge to
a liquid chromatography system, prepare it as follows
using Table 3 as a guide.
1. Set pump flow rate to 1.0 ml/min.
2. Wash the cartridge with degassed regeneration
buffer (G) for 10 minutes at 1.0 ml/min.
3. Wash the cartridge with degassed elution
buffer (D or E) for 10 minutes at 2.0 ml/min.
4. Wash the cartridge with degassed application
buffer (A, B, or C) for 10 minutes at 2.0 ml/min.
5. Equilibrate the cartridge with degassed
application buffer for 2 minutes at 1.0 ml/min.
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